ELISpot Plus: Human IL-27 (ALP)

ELISpot Plus: Human IL‑27 (ALP)

3458-4APW-2

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Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT27/361)
Detection mAbMT140, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-27 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-27 (ALP)
ApplicationELISpot
AnalyteIL-27
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-27.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
PBMC (100,000 cells/well) were incubated for 22 h in the absence or presence of LPS and recombinant human IFN-γ and the number of cells secreting IL-27 was analyzed by ELISpot. Spot analysis was made with Mabtech IRIS.

Learn how to combine Mabtech’s antibodies for the detection of human IL-12, IL-12/-23 (p40), IL-23, IL-27, and EBI3 in ELISA and ELISpot.

 

The IL-12 cytokine family

The IL-12 cytokine family consists of IL-12, IL-23, IL-27, IL-35, and IL-39. The existence of IL-39 in human remains, however, to be confirmed. The proteins are heterodimers of different subunits which are either unique or shared by several family members. IL-12 is a heterodimer, (p70), consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23 which is a heterodimer of p40 and a unique p19 subunit. The p40 subunit also exists as a monomer and homodimer. The EBI3 subunit is shared with IL-27, IL-35, and IL-39.

Mabtech supplies monoclonal antibodies with defined subunit specificities to p40, p35, p19, p28, and EBI3. Since both IL-12 and IL-23 heterodimers contain the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers. The EBI3 assays will detect IL-27, IL-35, and IL-39.

In our IL-12 and IL-23 assays, the same capture antibodies to p40 (MT86/221) are used. Different detection antibodies enable the detection of IL-12 (p70), IL-12/-23 (p40), and IL-23.

 

Application guide: mAbs to human IL-12, IL-23, IL-27 and EBI3

 

 IL-12 (p70) 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT704 (p70 specific)

     

 IL-23 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT155 (p19 specific)

     

 IL-27 
 
 
Capture mAbs: MT27/361 (IL-27 and p28 specific)
 
Detection mAb: MT140 (EBI3 specific)
 IL-12/-23 (p40) 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT618 (p40 specific)

     

 EBI3 (IL-27 and IL-35) 
 
Capture mAbs: MT23A14 (EBI3 specific)
 
Detection mAb: MT140 (EBI3 specific)

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's IL-23 ELISA, ELISpot, and FluoroSpot kits in everything from infectious disease research to novel immunotherapies. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-27

Analyte description

The cytokine interleukin 27 (IL-27) is a member of the IL-12 family. IL-27 is a heterodimer composed of one EBI3 subunit and one p28 subunit. Monocytes, endothelial cells, dendritic cells, and T cells produce it. IL-27 has been described to have both pro-inflammatory and anti-inflammatory properties. 

Alternative namesInterleukin 27, IL-27, IL27, IL-27AA, IL27p28, IL30, p28, IL-27B, IL27B, IL35B
Cell typeMonocyte/MΦ, mDC, NK cell
Gene ID246778, 10148